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Mitochondrial Compounds

NAD+

Also written nicotinamide adenine dinucleotide · NAD

NAD+ (β-nicotinamide adenine dinucleotide) is a dinucleotide coenzyme composed of nicotinamide mononucleotide joined to adenosine monophosphate. Supplied as a lyophilised powder in a sealed vial for laboratory research. Available in a 500 mg presentation.

In stock
500 mg vial£70.00

£0.14 per mg

≥99% purity (HPLC) · Independent third-party testing by PeptideVerify · COA Library

Order before 3pm for same-day dispatch.

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500 mg · £70.00 · £0.14/mg

At a glance

Research area
Mitochondrial Compounds · Explore the research area
Presentations
500 mg
Form
Lyophilised powder in a sealed vial
Purity
≥99% (HPLC)
CAS number
53-84-9
Molecular formula
C21H27N7O14P2
Molecular weight
663.43 g/mol
Dispatch
Order by 3pm Mon–Sat for same-day dispatch

About NAD+

NAD+ (β-nicotinamide adenine dinucleotide) is a dinucleotide coenzyme, not a peptide: it contains no amino acids. Its two nucleotides, nicotinamide mononucleotide and adenosine monophosphate, are joined through a pyrophosphate (diphosphate) linkage, and each is built on a D-ribose sugar. The positive charge in the name sits on the nitrogen of the nicotinamide ring, and NAD+ is the oxidised member of the NAD+/NADH redox pair.

The catalogue lists CAS number 53-84-9, the molecular formula C21H27N7O14P2 and a molecular weight of 663.43 g/mol. The adenine ring absorbs strongly at 260 nm, the usual wavelength for HPLC of NAD+, while NADH shows an additional band at 340 nm that distinguishes it spectroscopically. Nicotinamide and ADP-ribose are the expected breakdown products, and water content matters because the solid is hygroscopic. Each presentation is supplied as a lyophilised powder in a sealed vial.

Identifiers

Further reading

NAD+ structure and analytical profile

NAD+ is built from two nucleotides. Nicotinamide mononucleotide pairs a nicotinamide ring with a D-ribose and a phosphate; adenosine monophosphate pairs an adenine base with a second D-ribose and a phosphate. The two phosphates join in a pyrophosphate bridge, P–O–P, which links the halves into one molecule with the formula C21H27N7O14P2.

The β in the full name gives the configuration at the ribose carbon that carries the nicotinamide ring. Because the formula contains phosphorus and no amino acid, NAD+ is a nucleotide coenzyme, analysed with methods for nucleotides rather than peptide sequencing.

Each D-ribose is a five-carbon sugar in its furanose ring form, joined to its base through a glycosidic bond at C1′ and to its phosphate at C5′.

In NAD+ the nicotinamide ring is a pyridinium ion: aromatic, with a positive charge on its nitrogen. The NADH form carries an extra hydride on carbon 4 of that ring, which turns it into a 1,4-dihydropyridine and gives the formula C21H29N7O14P2, two hydrogens more, at 665.4 g/mol.

The plus sign belongs to the ring nitrogen, not to the whole molecule. The two phosphate groups of the bridge each carry a negative charge in water, so near neutral pH NAD+ has a net charge of −1. The adenine ring gives both forms an absorbance band near 260 nm, and only NADH adds a second band near 340 nm.

The monoisotopic mass is 663.1091 Da, so the protonated ion, [M+H]+, is expected at m/z 664.1164 in positive-mode electrospray. HPLC with detection at 260 nm gives purity as the main peak's share of total peak area and separates NAD+ from nicotinamide and ADP-ribose, its expected breakdown products, and from NADH.

In negative-mode electrospray the deprotonated ion, [M−H]−, is expected at m/z 662.1018, and the main fragments correspond to the loss of nicotinamide and to ADP-ribose, the same pieces that appear as breakdown products.

NAD+ is a white to off-white hygroscopic solid, so water content is part of its specification and the vial stays closed until it is needed. The sealed vial is stored at 2–8 °C short term, or at −20 °C and below for long-term storage, protected from light and away from repeated freeze-thaw cycles.

Buying NAD+ in the UK

Order NAD+ directly from British Peptide Labs, a UK-registered company, as a sealed vial in 500 mg presentation, from £70. Every size is listed below with its vial price and its price per mg, so the presentations compare at a glance.

NAD+ price (UK)
PresentationPrice£/mg
NAD+ 500 mg£70.00£0.14

Choose a presentation in the buy box, add it to your basket and check out. Orders placed before 3pm Mon–Sat are packed the same working day and handed to Royal Mail Tracked 24, and your tracking number is emailed as soon as the parcel leaves us. Larger orders are welcome: add as many vials as your laboratory needs in a single basket.

For what other UK suppliers charge, see our UK price per mg data, updated every month.

Product information

Synonym
nicotinamide adenine dinucleotide, NAD
CAS number
53-84-9
Molecular formula
C21H27N7O14P2
Molecular weight
663.43 g/mol
Appearance
White to off-white lyophilised powder
Purity
≥99% (HPLC)
Net content
500 mg per vial
Storage (sealed vial)
Store at 2–8 °C short term, or at −20 °C and below for long-term storage. Protect from light and avoid repeated freeze-thaw cycles
PubChem CID
5892
Research use
For laboratory research use only. Not for human or veterinary use.

NAD+ (β-nicotinamide adenine dinucleotide) is a dinucleotide coenzyme composed of nicotinamide mononucleotide joined to adenosine monophosphate. Supplied as a lyophilised powder in a sealed vial for laboratory research. Available in a 500 mg presentation. Store the sealed vial at 2–8 °C for short-term storage, or at −20 °C and below for long term. Protect from light and avoid repeated freeze-thaw cycles. For research use only. Not for human or veterinary use. Sold strictly for in-vitro laboratory research; not for diagnosis or treatment.

Packing
Each sealed vial travels in protective packing.
Dispatch
Orders placed before 3pm Mon–Sat leave the same day.
Delivery
Next-day tracked delivery with Royal Mail Tracked 24.
Tracking
Your tracking number is emailed as soon as the order is dispatched.
Delivery cost
Delivery is free on every order.
Shipping details

Questions about NAD+

NAD+ (β-nicotinamide adenine dinucleotide) is a dinucleotide coenzyme composed of nicotinamide mononucleotide joined to adenosine monophosphate. It is supplied in a sealed vial as a white to off-white lyophilised powder, in a 500 mg presentation.

Read the Learn article

NAD is the general abbreviation for nicotinamide adenine dinucleotide, and NAD+ names its oxidised form, with a positive charge on the nicotinamide ring. The vial holds NAD+, CAS number 53-84-9.

663.43 g/mol for the formula C21H27N7O14P2. The monoisotopic mass is 663.1091 Da, so the [M+H]+ ion is expected at m/z 664.1164 in positive-mode electrospray. In negative mode the [M−H]− ion is expected at m/z 662.1018.

53-84-9, the number CAS Common Chemistry records for β-nicotinamide adenine dinucleotide, with the formula C21H27N7O14P2. PubChem lists the compound as CID 5892.

About −1 near neutral pH. The nicotinamide ring nitrogen carries one positive charge, and the two phosphate groups of the pyrophosphate bridge carry one negative charge each.

NADH has two more hydrogen atoms, C21H29N7O14P2 at 665.4 g/mol, from the hydride on carbon 4 of the nicotinamide ring and an extra proton. That turns the aromatic pyridinium ring into a 1,4-dihydropyridine.

Its adenine ring absorbs strongly near 260 nm, which gives HPLC a clear signal for the main peak. NADH absorbs there too, but only NADH has a second band near 340 nm, so the two forms are easy to tell apart. Nicotinamide, a breakdown product, absorbs near 260 nm too, and HPLC separates it from the main peak.

The solid is hygroscopic and takes up water from air, which is why water content is part of its specification. The vial stays sealed at 2–8 °C short term, or at −20 °C and below for long-term storage, away from light.

Order NAD+ directly from British Peptide Labs, a UK-registered company. It is supplied in a sealed 500 mg vial at £70.00 (£0.14 per mg). The 500 mg vial is in stock. Every vial is ≥99% purity (HPLC), with independent third-party testing by PeptideVerify. Orders placed before 3pm Mon–Sat leave the same day by Royal Mail Tracked 24 for next-day tracked delivery.

NAD+ comes in one sealed vial: 500 mg at £70.00 (£0.14 per mg). Delivery is free on every order.

NAD+ is catalogued in the Mitochondrial Compounds research area, where compounds are grouped by molecular class. The research area page lists each of them with its identifiers.

Mitochondrial Compounds research area

NAD+ has independent third-party testing by PeptideVerify: reversed-phase HPLC measures purity, at ≥99% (HPLC), and LC-MS confirms identity against the expected mass.

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